Journal: Molecular Therapy. Methods & Clinical Development

Article Title: Comparing the efficacy of γ- and electron-irradiation of PBMCs to promote secretion of paracrine, regenerative factors

doi: 10.1016/j.omtm.2021.02.016

Figure Lengend Snippet: Cytokine secretion of γ- and electron-irradiated PBMCs (A) Immunodetection array membrane with annotations of the most abundant analytes detected in the secretomes of PBMCs. Each spot represents one analyte, and each analyte is spotted in duplicates. (B) Cytokine secretion profiles of 60 Gy γ- and 60 Gy electron-irradiated PBMCs. Pooled secretomes of n = 4 donors per condition were analyzed. (C) Analysis of integrated densities of chemiluminescent signals. Each column represents one analyte. Colors indicate fold changes of integrated intensities. (D) Pooled secretomes of n = 4 donors were measured by ELISA in technical triplicates. Bar diagrams show mean and standard deviation. For statistical analysis, two-tailed Mann-Whitney was performed. BAFF, B cell activating factor; BDNF, brain-derived neurotrophic factor; CFD, complement factor D; DPPIV, dipeptidyl-peptidase IV; EGF, epidermal growth factor; ENA-78, epithelial-derived neutrophil-activating protein 78; FGF19, fibroblast growth factor 19; ICAM1, intercellular adhesion molecule 1; IL1ra, interleukin 1 receptor antagonist; IL6, interleukin 6; IL8, interleukin 8; IL10, interleukin 10; IL11, interleukin 11; IL16, interleukin 16; IL17A, interleukin 17A; IL18BPa, interleukin 18-binding protein isoform a; IL22, interleukin 22; IL24, interleukin 24; MCP1, monocyte chemotactic protein 1; MIF, macrophage migration inhibitory factor; MIP3α, macrophage inflammatory protein 3 alpha; MMP9, matrix metallopeptidase 9; PDGF-AA, platelet-derived growth factor subunit A; PDGF-AB/BB, platelet-derived growth factor subunits A and B; PF4, platelet factor 4; RANTES, regulated and normal T cell expressed and secreted; RBP, retinol-binding protein; SDF1α, stromal cell-derived factor 1 alpha; PAI-1, plasminogen activator inhibitor-1; TFF3, trefoil factor 3; TIM3, T cell immunoglobulin and mucin-domain containing-3; uPAR, urokinase-type plasminogen activator receptor; VEGF, vascular endothelial growth factor; vitamin D BP, vitamin D-binding protein; TGFβ1, transforming growth factor beta 1.

Article Snippet: Cytokines were assessed by the Proteome Profiler Assay Human XL Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA), according to the manufacturer’s instructions.

Techniques: Irradiation, Immunodetection, Membrane, Enzyme-linked Immunosorbent Assay, Standard Deviation, Two Tailed Test, MANN-WHITNEY, Derivative Assay, Binding Assay, Migration